Topic particle 는: indicating a topic in a sentence, in this case meaning As for you, Jenny…
Subject particle 이: asking about new information, in this case, indicating that James does not know about Jenny's family.
Topic particle 는: marking a topic shift, in this case from 오빠 to 언니.
Particle 도: indicating a continuation of information, in this case meaning Jenny does not have an 오빠, and, neither does she have an 언니.
Particle 만: indicating the exclusivity of information, in this case indicating Jenny has one 남동생 only.
Subject particle 이: indicating new information and no comparison/ contrast at the discourse level.
Locative particle, 에: indicating static location that involves no action in the corresponding place.
Locative particle, 에서: indicating dynamic location, in this case the action of "studying."